After scoring in Liverpool’s 4-2 win, Mohamed Salah was in tears at full-time as Jota’s song reverberated around Anfield.
In his match programme notes, Liverpool boss Arne Slot said: “We know that this will be a very emotional occasion, given it is the first league game we have played since we lost Diogo and Andre.
“As I have said previously, the tributes that have been paid throughout the football world, and especially within the LFC community, have been truly special, and I know that tonight we will come together to honour them once more.
“It is important that, as a club, we show that they [Jota’s family] will always have our love and support as they deal with this most tragic of situations. We are there for them always.”
A moment of silence will be held at all Premier League matches this weekend, with players wearing black armbands, while messages and images will be shown on the big screens in stadiums.
Liverpool’s players will display their own tribute with a ‘Forever 20’ emblem printed on their shirts and stadium jackets. This will remain in place for the season.
The Reds won a record-equalling 20th English top-flight title last season with Jota wearing the number 20 shirt, which the club decided to retire after consulting with the former Portugal international’s wife Rute Cardoso and family members.
Jota’s former club Wolves also have tributes planned for their season opener at home to Manchester City on Saturday.